Studies on the Growth in Culture of Plant Cells: VIII. THE PRODUCTION OF ETHYLENE BY SUSPENSION CULTURES OFACER PSEUDOPLATANUS, L.

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Abstract
Sycamore cell suspension cultures in a synthetic medium release ethylene; during a 24-day incubation period a single culture (initial volume 70 ml) produces c. 4 μ moles. There is a very sharp peak of ethylene production between day 10 and day 14 of culture; at the peak of production c. 2 nmoles ethylene are released per million cells in 24 h. Evidence is presented that 2,4-D enhances ethylene production independently of its effects on culture growth. Under the standard conditions of culture (250-ml Erlenmeyer flasks closed with aluminium foil and containing 70 ml cell suspension) the concentration of ethylene in the gas phase of the cultures rises above 10 ppm. No evidence was obtained that this ethylene is inhibitory to culture growth or that a critical level of ethylene is necessary to initiate cell division in cultures at a critically low cell density. The low rate of ethylene release by stationary phase cultures is temporarily enhanced by the addition of various solutes and further depressed by dilution with water.

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