Biochemical analysis of bovine testicular anti‐Müllerian hormone
- 20 January 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 195 (1-2) , 73-76
- https://doi.org/10.1016/0014-5793(86)80133-8
Abstract
Direct biochemical analysis has been applied to bovine testicular anti-Müllerian hormone (AMH), purified from incubation medium of bovine fetal testes by immunochromatography on a monoclonal antibody. The hormone contains a high proportion of hydrophobic amino acids and 13.5% carbohydrate. The oligosaccharide composition suggests that both N- and O-glycosidically linked chains are present. The molecular extinction coefficient is 3.27 ± 0.06. One RIA unit, defined as the amount of hormone released by 1 g fetal bovine testicular tissue incubated during 4 h, corresponds to 3.06 ± 0.17 /smg proteinKeywords
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