Comparison of immunoadsorbents prepared by coupling sperm-whale myoglobin to a variety of insoluble polymers
- 1 November 1970
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 120 (2) , 373-379
- https://doi.org/10.1042/bj1200373
Abstract
Immunoadsorbents that were potentially suitable for the fractionation of antibodies to myoglobin were prepared by coupling myoglobin to aminoethylcellulose, bromoacetylcellulose, CM-cellulose, an ethylene–maleic anhydride co-polymer and Sepharose 4B. A comparison of the properties of the preparations indicated that Sepharose–myoglobin was superior, as an immunoadsorbent, to the other conjugates. Elution of adsorbed antibodies with m-propionic acid, pH2.5, gave yields of precipitable antibody higher than those achieved with other dissociating solvents. Propionic acid eluted 60–100% of the antibody adsorbed by Sepharose–myoglobin, and 60–90% of the eluted protein was precipitable antibody.Keywords
This publication has 20 references indexed in Scilit:
- The chemical attachment of chymotrypsin to water-insoluble polymers using
amino -4,6-dichloro -s-Triazine Biochimica et Biophysica Acta (BBA) - Enzymology, 1970 - Carrier-bound proteins: Properties of peroxidase bound to insoluble carboxymethylcellulose particlesArchives of Biochemistry and Biophysics, 1969
- Isolation of antibodies to the C-terminal heptapeptide of myoglobin with a synthetic peptideImmunochemistry, 1968
- Chemical Coupling of Peptides and Proteins to Polysaccharides by Means of Cyanogen HalidesNature, 1967
- Purification of antibodies with immunoadsorbents prepared using bromoacetyl celluloseImmunochemistry, 1967
- Water-Insoluble Derivatives of Enzymes, Antigens, and AntibodiesAnnual Review of Biochemistry, 1966
- A Water-insoluble Polyanionic Derivative of Trypsin. I. Preparation and Properties*Biochemistry, 1964
- Use of antibody bound to modified cellulose as an immunospecific adsorbent of antigensBiochimica et Biophysica Acta, 1963
- Simplified methods for the estimation of iron in mitochondria and submitochondrial fractionsArchives of Biochemistry and Biophysics, 1962
- Synthesis of Biologically Active Cellulose Derivatives of EnymesNature, 1961