Separation of Carboxy- and Methemoglobin by Cation Exchange Dextran Gels
- 1 January 1963
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 15 (4) , 331-338
- https://doi.org/10.3109/00365516309079753
Abstract
Human adult carboxyhemoglobin (Hb,-Co), methemoglobin (MHb,), and mixtures of the pure derivatives were transferred into phosphate buffer pH 6.0 by gel filtration on Sephadex G 25 and then adsorbed on two different ion exchangers with a high degree of swelling. In one case the charged groups were carboxyl, CM-Sephadex C-50, and in the other sulphonate, SE-Sephadex C-50. They were then eluted from the column with a continuously increasing concentration of NaCl in the same buffer. On CM-Sephadex, HbA CO separated into two fractions, probably HbA0, CO and HbA1CO, but this fractionation was poor on SE-Sephadex. Mixtures of Hb, CO and hfHb, gave a good separation on SE-Sephadex, less good on CM-Sephadex. A two-step elution procedure gave a good separation on SE-Sephadex with purities of over 90 per cent in parts of both fractions. The elution behavior indicated that the same result can be obtained by elution with a constant eluant concentration.Keywords
This publication has 2 references indexed in Scilit:
- The separation of different types of human haemoglobinJournal of Chromatography A, 1959
- New Hemoglobin in Normal Adult BloodScience, 1955