Na+‐independent Mg2+ efflux from Mg2+‐loaded human erythrocytes
- 24 April 1989
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 247 (2) , 181-184
- https://doi.org/10.1016/0014-5793(89)81329-8
Abstract
Net Mg2+ efflux from Mg2+‐loaded human erythrocytes was maximal after reincubation in sucrose. Net Mg2+ efflux was not inhibited by furosemide or bumetanide and, therefore, was not performed by the (Na,K,Cl)‐ or (K,Cl)‐cotransport system. A component of net Mg2+ efflux was inhibited by extracellular NaCl, KCl, LiCl, choline Cl and SITS, in analogy to the inhibition of net Cl− and SITS. Therefore, it was concluded that net Mg2+ efflux is dependent on net Cl− efflux for charge compensation. Cl−‐dependent net Mg2+ efflux was inhibited by amiloride. Only 10% of the maximal net Mg2+ efflux may depend on extracellular Na+.Keywords
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