STIMULATION OF DIFFERENTIATION OF MOUSE MYELOID LEUKEMIC-CELLS AND INDUCTION OF INTERFERON IN THE CELLS BY DOUBLE-STRANDED POLYRIBONUCLEOTIDES

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 39  (10) , 4170-4174
Abstract
Mouse myeloid leukemic Ml cells can be induced to differentiate into mature macrophages and granulocytes by differentiation-stimulating factor (D-factor) in conditioned medium of mouse peritoneal macrophages. Double-stranded RNA, such as the copolymers of polyinosinic and polycytidylic acids and polyadenylic and polyuridylic acids, could not alone induce differentiation of the cells, but enhanced induction of differentiation by low concentrations of the D-factor and induced a significant amount of interferon. Rabbit antiserum to purified [mouse fibroblast] L-cell interferon neutralized the antiviral activity of interferon of Ml cells. Simultaneous treatment of Ml cells with the anti-interferon serum and copolymer and polyinosine and polycytidylic acids and D-factor abolished the enhancing effect of copolymer of polyinosine and polycytidylic acids on the action of the D-factor. The effect of double-stranded RNA on induction of differentiation of Ml cells may be mediated by interferon produced by the cells.