STUDIES OF THE CYTOCHEMISTRY OF NUCLEOPROTEINS II. IMPROVED STAINING METHODS WITH TOLUIDINE BLUE AND AMMONIUM MOLYBDATE

Open Access
Publisher Website
Google Scholar
Abstract
Staining of the nucleoproteins of various types of cancer cells by toluidine blue- molybdate (TBM) depends upon graded inactivation of protein-bound amino groups or alteration in the concentrations and time of reaction of the dye and molybdate to allow the formation of a nucleic acid-toluidine blue complex that is capable of reacting with polymolybdates to produce a metachromatic polymer. Toluidine blue samples of high purity differed from older dyes in the nature of dye binding and in the rate of formation of the nucleic acid-dye complex that reacts with molybdate. The improved TBM procedures stain deoxyribonucleoprotein and 9 types of ribonucleoprotein (RNP) in the cell. The characteristics of the types of RNP are reviewed. The results suggest that, as the RNA which is synthesized in the chromatin and chromosomes diffuses to the perichromosomal and interchromosomal regions, it is functionally and molecularly changed by virtue of association with amino-acids and protein.

This publication has 5 references indexed in Scilit: