CDP-glycerol:poly(glycerophosphate) glycerophosphotransferase, which is involved in the synthesis of the major wall teichoic acid in Bacillus subtilis 168, is encoded by tagF (rodC)
Open Access
- 1 January 1992
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 174 (2) , 646-649
- https://doi.org/10.1128/jb.174.2.646-649.1992
Abstract
Assays of CDP-glycerol:poly(glycerophosphate) glycerophosphotransferase (CGPTase) (EC 2.7.8.12) in membranes isolated from Bacillus subtilis 168 wild type and 11 strains bearing conditional lethal thermosensitive mutations in tagB, tagD, or tagF revealed that CGPTase deficiency was associated only with mutant tagF alleles. In vitro, thermosensitivity of CGPTase strongly suggests that the structural gene for this enzyme is tagF. We discuss apparent discrepancies between biochemical evidence favoring a membrane location for TagF and a previous report that suggested a cytoplasmic location based on sequence analysis.Keywords
This publication has 13 references indexed in Scilit:
- Genetic and biochemical characterization of Bacillus subtilis 168 mutants specifically blocked in the synthesis of the teichoic acid poly(3-O- -D-glucopyranosyl-N-acetylgalactosamine 1-phosphate): gneA, a new locus, is associated with UDP-N-acetylglucosamine 4-epimerase activityJournal of General Microbiology, 1991
- Genes concerned with synthesis of poly(glycerol phosphate), the essential teichoic acid in Bacillus subtilis strain 168, are organized in two divergent transcription unitsJournal of General Microbiology, 1991
- A conditional-lethal mutant of Bacillus subtilis 168 with a thermosensitive glycerol-3-phosphate cytidylyltransferase, an enzyme specific for the synthesis of the major wall teichoic acidJournal of General Microbiology, 1991
- The nucleotide sequence of the rodC operon of Bacillus subtilisMolecular Microbiology, 1989
- The essential nature of teichoic acids in Bacillus subtilis as revealed by insertional mutagenesisMolecular Genetics and Genomics, 1989
- The gtaB Marker in Bacillus subtilis 168 Is Associated with a Deficiency in UDPglucose PyrophosphorylaseMicrobiology, 1987
- Biosynthesis of the unit that links teichoic acid to the bacterial wall: Inhibition by tunicamycinFEBS Letters, 1976
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Solubilisation of a teichoic acid‐synthesising system from the membrane of Bacillus licheniformis by freezing and thawingFEBS Letters, 1973