Studies on the Acid-protease ofPaecilomyces variotiBainier TPR-220

1 October 1963

journal article
research article
Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry

Vol. 27 (10) , 677-683
https://doi.org/10.1080/00021369.1963.10858167

Abstract

A fungus, Paecilomyces varioti Bainier TPR-220 was found to produce an acid-protease on wheat bran culture (Koji). The protease showed the highest activity at pHs between 2.5 and 3.0 on casein as substrate. The enzyme was stable at pHs from 2.5 to 6.0 at 40°C for forty minutes. By crystallization from 50% acetone solution the specific activity of the protease was increased five times as high as that of the original crude enzyme preparation.