Effect of temperature and pH on the hopanoid content of Bacillus acidocaldarius
- 1 August 1984
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 23 (2-3) , 253-256
- https://doi.org/10.1016/0378-1097(84)90219-2
Abstract
A gas-liquid chromatographic method for the quantitative determination of hopanoids with an elongated side chain was developed. After extraction of lipids from Bacillus acidocaldarius , periodate oxidation, reduction and acetylation the 1-hydroxyethane-29-hopane acetate was quantitated by comparison with 1-octadecanyl-glycerol ether. The contents of extended hopanoids increased strongly with increasing temperature and moderately with decreasing pH. Hopene showed no significant alteration with temperature and pH.Keywords
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