Rapid isolation of bacteroids from soybean root nodules by percoll discontinuous gradient centrifugation
Open Access
- 1 June 1989
- journal article
- research article
- Published by Taylor & Francis in Soil Science and Plant Nutrition
- Vol. 35 (2) , 301-305
- https://doi.org/10.1080/00380768.1989.10434762
Abstract
A method for the rapid isolation of bacteroids from soybean (Glycine max L. Merr.) root nodules is described. Bacteroids were isolated by Percoll discontinuous gradient centrifugation. Assays of marker enzymes and observation by electron microscopy revealed that the bacteroids obtained were essentially free from contamination by plant cell organelles. The yield of pure bacteroids from 1 g of fresh nodules was approximately 60 mg of bacteroid protein.Keywords
This publication has 6 references indexed in Scilit:
- Metabolism of [13C]‐labelled photosynthate in plant cytosol and bacteroids of root nodules of Glycine maxPhysiologia Plantarum, 1986
- Effects of oxygen concentration and leghemoglobin on organic acid degradation by isolated soybean nodule bacteriods.Agricultural and Biological Chemistry, 1985
- Isolation and Characterization of Infected and Uninfected Cells from Soybean NodulesPlant Physiology, 1983
- A rapid one-step method for the isolation of bacteroids from root nodules of soybean plants, utilizing self-generating Percoll gradientsCanadian Journal of Microbiology, 1981
- Isolation of Bacteria, Transforming Bacteria, and Bacteroids from Soybean NodulesPlant Physiology, 1977
- Poly-β-hydroxybutyrate Utilization by Soybean (Glycine max Merr.) Nodules and Assessment of Its Role in Maintenance of Nitrogenase ActivityPlant Physiology, 1971