A Novel Strategy for Analysis of Gene Homologues and Segmental Genome Duplications
- 1 June 2003
- journal article
- research article
- Published by Springer Nature in Journal of Molecular Evolution
- Vol. 56 (6) , 702-710
- https://doi.org/10.1007/s00239-002-2442-x
Abstract
Transformation-associated recombination (TAR) cloning allows selective isolation of a desired chromosomal region or gene from complex genomes. The method exploits a high level of recombination between homologous DNA sequences during transformation in the yeast Saccharomyces cerevisiae. We investigated the effect of nonhomology on the efficiency of gene capture and found that up to 15% DNA divergence did not prevent efficient gene isolation. Such tolerance to DNA divergence greatly expands the potential applications of TAR cloning for comparative genomics. In this study, we were able to use the technique to isolate nonidentical chromosomal duplications and gene homologues.Keywords
This publication has 32 references indexed in Scilit:
- Functional evidence for a metastasis suppressor gene for rat prostate cancer within a 60-kilobase region on human chromosome 8p21-p12.2002
- Linker-Mediated Recombinational Subcloning of Large DNA Fragments Using YeastGenome Research, 2002
- Radial Transformation-Associated Recombination Cloning from the Mouse Genome: Isolation of Tg.AC Transgene with Flanking DNAsGenomics, 2000
- Sequences flanking the centromere of human chromosome 10 are a complex patchwork of arm-specific sequences, stable duplications and unstable sequences with homologies to telomeric and other centromeric locationsHuman Molecular Genetics, 1999
- Dual roles for DNA sequence identity and the mismatch repair system in the regulation of mitotic crossing-over in yeastProceedings of the National Academy of Sciences, 1997
- Minimum Length of Sequence Homology Required forin VivoCloning by Homologous Recombination in YeastPlasmid, 1997
- Direct isolation of human BRCA2 gene by transformation-associated recombination in yeastProceedings of the National Academy of Sciences, 1997
- Transformation‐associated recombination between diverged and homologous DNA repeats is induced by strand breaksYeast, 1994
- Recombination between similar but not identical DNA sequences during yeast transformation occurs within short stretches of identityCell, 1992
- Protein engineering by cDNA recombination in yeasts: shuffling of mammalian cytochrome P-450 functionsGene, 1989