Nearest neighbor analysis of D1 and D2 subunits in the photosystem II reaction center using a bifunctional cross‐linker, hexamethylene diisocyanate
- 29 August 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 351 (1) , 27-30
- https://doi.org/10.1016/0014-5793(94)00803-5
Abstract
A cross-linked product between the D1 and D2 subunits was generated by treating isolated spinach PS II reaction center with a bifunctional cross-linker, 1,6-hexamethylene diisocyanate. To obtain information on the contact site(s) between D1 and D2 proteins, the cross-linked product was cleaved by CNBr, the resultant fragments were separated using a reverse-phase HPLC, and then the partial sequence of the cross-linked polypeptide fragments were determined. By comparing the results with the deduced amino acid sequence of the D1 and D2 proteins from spinach, it is concluded that the C-terminal domains of the D1 subunit (D308-A334) and that of the D2 subunit (Y297-L353) are in close proximityKeywords
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