Intracellular Distribution of Steroid Glycosidases of Rabbit Liver

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Abstract
The activity and intracellular distribution of several glycosidases in rabbit liver have been examined. Glycosides of the steroid estrogens, which have been shown to occur naturally in the rabbit, were used as substrates and the results were compared to those obtained when synthetic p-nitrophenyl glycosides were used. β-N-acetylglucosaminidase (EC 3.2.1.30) and β-glucuronidase (EC 3.2.1.31) activities were concentrated in the lysosomes and showed the latency typical of lysosomal acid hydrolases. β-D-Glucosidase (EC 3.2.1.21) activity was concentrated in the unsedimentable fraction of the liver homogenates and did not exhibit latency. No differences were found in the intracellular distribution of glycosidase activities towards the steroid and the p-nitrophenyl glycosides.

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