Penetratin‐induced aggregation and subsequent dissociation of negatively charged phospholipid vesicles
Open Access
- 31 August 2001
- journal article
- Published by Wiley in FEBS Letters
- Vol. 505 (2) , 307-312
- https://doi.org/10.1016/s0014-5793(01)02843-5
Abstract
The interaction of the cellular delivery vector penetratin with a model system consisting of negatively charged phospholipid vesicles has been studied. Above a certain peptide to lipid molar ratio, the cationic oligopeptide induces vesicle aggregation. Interestingly, the aggregation is followed by spontaneous disaggregation, which may be related to membrane translocation of the peptide. Circular dichroism (CD) measurements indicate a conformational transition, from α‐helix to antiparallel β‐pleated sheet, which is simultaneous with the aggregation process. The potential influence of spectroscopic artifacts on CD data due to the drastically increased turbidity during aggregation is discussed.Keywords
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