The Effect of High pH and Calcium on Tobacco Leaf Protoplast Fusion
Open Access
- 1 December 1973
- journal article
- research article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 28 (11-12) , 737-741
- https://doi.org/10.1515/znc-1973-11-1215
Abstract
High frequencies of fusion were induced between isolated tobacco leaf protoplasts when they were incubated at 37 °C in the presence of 0.05 M CaCl2 in 0.4 ᴍ mannitol at pH 10.5. Subsequent to the fusion treatment the protoplasts were washed and cultured in a suitable medium and within two weeks, actively dividing cell colonies were observed. The fusion treatment had no irreversible, deleterious effects on the protoplastsKeywords
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