T lymphocyte-mediated cytolysis. I. A common mechanism for target recognition in specific and lectin-dependent cytolysis.
Open Access
- 1 August 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 127 (2) , 776-781
- https://doi.org/10.4049/jimmunol.127.2.776
Abstract
In this and the accompanying paper we examine the nature of the interactions between effector cells and target cells leading to lysis in T cell-mediated cytolysis reactions. In the first paper, we re-examine the role of lectin (Con A) in the process of lectin-dependent cell-mediated cytotoxicity (LDCC). Lectin has generally been thought to act simply as a bridge between the cytotoxic effector cell (EC) and the target cell (TC), thus bypassing the need for receptor-antigen interaction and accounting for the nonspecific character of this lytic reaction. A role for lectin in the activation of cytotoxic function in the EC has also been suggested. In this paper we confirm that LDCC occurs when TC alone are pretreated with lectin, but not when EC alone are pretreated with lectin, unless free lectin is also present in the assay mixture. In addition, we demonstrate that the failure of lectin-pretreated EC to bind to and lyse TC is not caused by agglutination or self-destruction of the EC. Moreover, lectin-pretreated EC are not inherently deficient in the ability to bind or lyse TC, if the latter are pretreated with lectin, or if free lectin is present in the assay. Finally, when EC are used as both effectors and targets in LDCC, lysis is observed only in the direction of the lectin-pretreated partner. From these results we conclude that in LDCC, as in specific CTL cytolysis, CTL-TC recognition proceeds through interaction of an EC surface receptor (distinct from the lectin receptor), and a TC antigen perhaps modified by, but distinct from, the lectin itself. In the accompanying paper we present evidence that this receptor-determinant interaction involves TC MHC antigen.This publication has 11 references indexed in Scilit:
- Enumeration of T-effector cells mediating direct and/or lectin-dependent lysisImmunology Letters, 1980
- Inhibition of cytotoxic T lymphocytes with concanavalin ACellular Immunology, 1980
- Lectin-induced nonlethal adhesions between cytolytic T lymphocytes and antigenically unrecognizable tumor cells and nonspecific "triggering" of cytolysis.The Journal of Immunology, 1980
- Lectin-dependent cell-mediated cytotoxicity: Induction of a unique effector cell populationCellular Immunology, 1978
- STUDIES ON THE INDUCTION AND EXPRESSION OF T CELL-MEDIATED IMMUNITYTransplantation, 1978
- T Lymphocyte-Mediated Cytolysis: Dissociation of the Binding and Lytic Mechanisms of the Effector CellThe Journal of Immunology, 1978
- STUDIES ON MECHANISM OF LYMPHOCYTE-MEDIATED CYTOLYSIS .11. ROLE OF LECTIN IN LECTIN-DEPENDENT CELL-MEDIATED CYTOTOXICITY1978
- Comparative Studies on the Mechanisms of Nonspecific, Con A-Dependent Cytolysis and Specific T Cell-Mediated CytolysisThe Journal of Immunology, 1977
- STUDIES ON MECHANISM OF LYMPHOCYTE-MEDIATED CYTOLYSIS .8. USE OF CON A TO DELINEATE A DISTINCTIVE KILLER T CELL SUBPOPULATION1977
- STUDIES ON MECHANISM OF LYMPHOCYTE-MEDIATED CYTOLYSIS .9. RELATIONSHIPS BETWEEN ANTIGEN RECOGNITION AND LYTIC EXPRESSION IN KILLER T CELLS1977