Selection methods in bacilli for recombinants and transformants of intra- and interspecific fused protoplasts
- 1 July 1983
- journal article
- research article
- Published by Springer Nature in Archiv für Mikrobiologie
- Vol. 134 (4) , 303-308
- https://doi.org/10.1007/bf00407807
Abstract
The intraspecific fusion frequencies obtained with the direct selection method on a semi-synthetic regeneration medium between strains of B. subtilis and B. licheniformis were distribution from 9.9×10-2 to 4.5×10-3, which was one or two orders higher than those of interspecific recombinations between B. subtilis and B. licheniformis. The regeneration media were also useful for selecting interspecific transformants from plasmid carrier to non-carrier. This selection could be used as a primary selection method for inter-and intraspecific recombinants obtained by protoplast fusion.Keywords
This publication has 20 references indexed in Scilit:
- Genetic Recombination in Fused Spheroplasts of Providence alcalifaciensJournal of General Microbiology, 1979
- Polyethylene-glycol induced fusion of bacterial protoplastsMolecular Genetics and Genomics, 1979
- Genetic Recombination in Streptomyces fradiae by Protoplast Fusion and Cell RegenerationJournal of General Microbiology, 1978
- Deoxyribonucleic Acid Homology and Taxonomy of the Genus BacillusInternational Journal of Systematic and Evolutionary Microbiology, 1978
- Genetic recombination through protoplast fusion in StreptomycesNature, 1977
- Fusion of bacterial protoplasts.Proceedings of the National Academy of Sciences, 1976
- Fusion of protoplasts of Bacillus megaterium.Proceedings of the National Academy of Sciences, 1976
- Taxonomic Study of Bacillus by Deoxyribonucleic Acid-Deoxyribonucleic Acid Hybridization and Interspecific TransformationInternational Journal of Systematic and Evolutionary Microbiology, 1975
- Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose-ethidium bromide electrophoresisBiochemistry, 1973
- Gene conservation in Bacillus species. I. Conserved genetic and nucleic acid base sequence homologies.Proceedings of the National Academy of Sciences, 1965