Nucleotide sequence of the ugp genes of Escherichia coli K‐12: homology to the maltose system

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Abstract
The nucleotide sequence of the ugp genes of Escherichia xoli K-12, which encode a phosphate-limitation inducible uptake system for sn-blycerol-3-phosphate and glycerophosphoryl diesters, was determined. THe genetic organization of the operon differed from previously published results. A single promoter, containing a putative pho box, was detected by S1-nuclease mapping. The promoter is followed by four open reading frames, designated ugPB, A, E and C, which encode a periplasmic binding protein, two hydrophobic membrane proteins and a protein that is likely to couple energy to the transport system, respectively. The sequences of the proteins contain the characteristics of several other binding protein-dependent transport systems, but they seem to be particularly closely related to the maltose system.