Abstract
The synthesis of the 2,4-diamino analogue of riboflavin [potential tumor chemotherapeutic agent] is described. Inhibition analysis in a microbial assay system [Lactobacillus leichmannii] indicated that this compound has a weak antifolate activity that could be overcome with a minimal amount of folinic acid, but at higher concentrations both folinic acid and riboflavin were required for the reversal of its inhibitory effect.

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