Cardiac and skeletal muscle mitochondria have a monocarboxylate transporter MCT1
- 1 November 1999
- journal article
- research article
- Published by American Physiological Society in Journal of Applied Physiology
- Vol. 87 (5) , 1713-1718
- https://doi.org/10.1152/jappl.1999.87.5.1713
Abstract
To evaluate the potential role of monocarboxylate transporter-1 (MCT1) in tissue lactate oxidation, isolated rat subsarcolemmal and interfibrillar cardiac and skeletal muscle mitochondria were probed with an antibody to MCT1. Western blots indicated presence of MCT1 in sarcolemmal membranes and in subsarcolemmal and interfibrillar mitochondria. Minimal cross-contamination of mitochondria by cell membrane fragments was verified by probing for the sarcolemmal protein GLUT-1. In agreement, immunolabeling and electron microscopy showed mitochondrial MCT1 in situ. Along with lactic dehydrogenase, the presence of MCT1 in striated muscle mitochondria permits mitochondrial lactate oxidation and facilitates function of the “intracellular lactate shuttle.”Keywords
This publication has 22 references indexed in Scilit:
- Cloning and sequencing of four new mammalian monocarboxylate transporter (MCT) homologues confirms the existence of a transporter family with an ancient pastBiochemical Journal, 1998
- Metabolism of [3-13C]pyruvate and [3-13C]propionate in normal and ischaemic rat heart in vivo: 1H- and 13C-NMR studiesBiochemical Journal, 1995
- cDNA cloning of MCT1, a monocarboxylate transporter from rat skeletal muscleBiochimica et Biophysica Acta (BBA) - Biomembranes, 1995
- Molecular characterization of a membrane transporter for lactate, pyruvate, and other monocarboxylates: Implications for the Cori cycleCell, 1994
- The Mitochondrial Carrier Family of Transport Proteins: Structural, Functional, and Evolutionary RelationshipsCritical Reviews in Biochemistry and Molecular Biology, 1993
- Lactate transport is mediated by a membrane-bound carrier in rat skeletal muscle sarcolemmal vesiclesArchives of Biochemistry and Biophysics, 1990
- Myocardial substrate utilization during exercise in humans. Dual carbon-labeled carbohydrate isotope experiments.Journal of Clinical Investigation, 1988
- Lactate:Glycolytic End Product and Oxidative Substrate During Sustained Exercise in Mammals — The “Lactate Shuttle”Proceedings in Life Sciences, 1985
- Biochemical adaptation of mitochondria, muscle, and whole-animal respiration to endurance trainingArchives of Biochemistry and Biophysics, 1981
- Specific inhibition of pyruvate transport in rat liver mitochondria and human erythrocytes by α-cyano-4-hydroxycinnamate (Short Communication)Biochemical Journal, 1974