Isolation of nit-4, the minor nitrogen regulatory gene which mediates nitrate induction in Neurospora crassa
- 1 July 1989
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 171 (7) , 4067-4070
- https://doi.org/10.1128/jb.171.7.4067-4070.1989
Abstract
Expression of nitrate reductase in Neurospora crassa requires the positive action of nit-4, a pathway-specific regulatory gene, which mediates nitrate induction. We report the molecular cloning of the nit-4 gene and present results which suggest that the nit-4 gene is constitutively expressed to yield a low-abundance 2.2-kilobase transcript. These results indicate that the nit-2 major control gene and the nit-4 pathway-specific control gene independently regulate the expression of the nitrate assimilatory structural genes. ImagesThis publication has 25 references indexed in Scilit:
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