EFFECT OF ADRIAMYCIN ON CELL-CYCLE PROGRESSION AND DNA-REPLICATION IN CHINESE-HAMSTER OVARY CELLS

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 37  (1) , 200-205
Abstract
Chinese hamster ovary cells were treated with the antitumor agent adriamycin (ADR) at various stages of the cell cycle to determine the effect on cell cycle progression and subsequent DNA replication. Concentrations of ADR up to 5 .mu.g/ml for 15 min produced no progression delay in cells treated 1 h prior to mitosis [M) and concentrations of 2 .mu.g/ml or lower had no effect on progression to M of G2 or late S phase cells. Cells treated in mid-S phase showed a significant, dose dependent delay at concentrations as low as 0.5 .mu.g/ml. This cell cycle effect on progression was also reflected in the rates of DNA replication in the subsequent S phase. DNA replication was not inhibited by 5 .mu.g/ml for 30 min when applied in the G1, M or G2 phase. When ADR was applied in the S or G1 phases of the previous cell cycle, a marked depression in the rate of replication of DNA was observed. This was the case of DNA synthesized before and after ADR treatment.