HUMAN PARATHYROID HORMONE CARBOXYTERMINAL PEPTIDE (53-84) STIMULATES ALKALINE PHOSPHATASE ACTIVITY IN DEXAMETHASONE-TREATED RAT OSTEOSARCOMA CELLS IN VITRO
- 1 February 1989
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 124 (2) , 1097-1099
- https://doi.org/10.1210/endo-124-2-1097
Abstract
Previous studies in our laboratory have demonstrated relatively large numbers of cell surface binding sites for the carboxyterminal (52-84) region of PTH on ROS 17/2.8 rat osteosarcoma cells, a clonal osteoblast-like cell line. In order to gain insight into the significance of these carboxyterminal binding the human PTH carboxyterminal fragment (52-84) on alkaline phosphatase activity in dexamethasone-treated rat osteosarcoma (ROS) 17/2.8 cells. While bovine PTH (1-84) and its aminoterminal 1-34 fragment inhibited alkaline phosphatase activity, we saw a dose-related stimulation of activity by human PTH (53-84), with maximal stimulation occurring after 120 hours, at a concentration of 10-8 M. The efect was not seen in dexamethasone-untreated cells.This publication has 7 references indexed in Scilit:
- Binding of Intact Parathyroid Hormone to Chicken Renal Plasma Membranes: Evidence for a Second Binding Site with Carboxyl-Terminal Specificity*Endocrinology, 1985
- Binding of Intact Parathyroid Hormone to Rat Osteosarcoma Cells: Major Contribution of Binding Sites for the Carboxyl-Terminal Region of the Hormone*Endocrinology, 1985
- Glucocorticoid Regulation of Alkaline Phosphatase in the Osteoblastic Osteosarcoma Cell Line ROS 17/2.8*Endocrinology, 1985
- Binding of Radioiodinated Parathyroid Hormone to Cloned Bone Cells*Endocrinology, 1983
- Binding of Radioiodinated Bovine Parathyroid Hormone-(1–84) to Canine Renal Cortical Membranes*Endocrinology, 1983
- Identification of a Receptor-Binding Region in Parathyroid Hormone*Endocrinology, 1980
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951