Control of cAMP-regulated enhancers by the viral transactivator Tax through CREB and the co-activator CBP
- 18 April 1996
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 380 (6575) , 642-646
- https://doi.org/10.1038/380642a0
Abstract
THE Tax protein of human T-lymphotrophic virus (HTLV)-l activates expression of the HTLV-1 long terminal repeat through a DNA element that resembles the cellular cyclic AMP-regulated enhancer (CRE)1,2. Tax contains a transcriptional activation domain3, but its ability to activate gene expression depends on interactions with cellular CRE-binding proteins such as CREB. Whether Tax can activate the expression of cellular CRE-contain-ing genes has been controversial. Here we show that Tax can activate both the HTLV-1 and consensus cellular CREs, and propose that this activation may occur through mechanisms that are differentially dependent on CREB phosphorylation. Tax not only increases the binding of CREB to the viral CRE but also recruits the transcriptional co-activator CBP4,5 in a manner independent of CREB phosphorylation. In contrast, association of Tax with the cellular CRE occurs through CBP which, in turn, is recruited only in the presence of phosphorylated CREB.Keywords
This publication has 24 references indexed in Scilit:
- A Molecular Mechanism for Human T-cell Leukemia Virus Latency and Tax TransactivationPublished by Elsevier ,1995
- Nuclear protein CBP is a coactivator for the transcription factor CREBNature, 1994
- In vitro selection of DNA elements highly responsive to the human T-cell lymphotropic virus type I transcriptional activator, Tax.Molecular and Cellular Biology, 1994
- Phosphorylated CREB binds specifically to the nuclear protein CBPNature, 1993
- Human T-cell lymphotropic virus type I (HTLV-I) transcriptional activator, Tax, enhances CREB binding to HTLV-I 21-base-pair repeats by protein-protein interaction.Proceedings of the National Academy of Sciences, 1992
- Type I human T cell leukemia virus tax protein transforms rat fibroblasts through the cyclic adenosine monophosphate response element binding protein/activating transcription factor pathway.Journal of Clinical Investigation, 1991
- Cis-acting sequences responsible for the transcriptional activation of human T-cell leukemia virus type I constitute a conditional enhancer.Proceedings of the National Academy of Sciences, 1986
- Location of cis-acting regulatory sequences in the human T-cell leukemia virus type I long terminal repeat.Proceedings of the National Academy of Sciences, 1985
- The pX Protein of HTLV-I Is a Transcriptional Activator of Its Long Terminal RepeatsScience, 1985
- Functional activation of the long terminal repeat of human T-cell leukemia virus type I by a trans-acting factor.Proceedings of the National Academy of Sciences, 1985