A Riboswitch Regulates Expression of the Coenzyme B 12 -Independent Methionine Synthase in Mycobacterium tuberculosis : Implications for Differential Methionine Synthase Function in Strains H37Rv and CDC1551
- 1 May 2007
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 189 (9) , 3655-3659
- https://doi.org/10.1128/jb.00040-07
Abstract
We observed vitamin B(12)-mediated growth inhibition of Mycobacterium tuberculosis strain CDC1551. The B(12) sensitivity was mapped to a polymorphism in metH, encoding a coenzyme B(12)-dependent methionine synthase. Vitamin B(12)-resistant suppressor mutants of CDC1551 containing mutations in a B(12) riboswitch upstream of the metE gene, which encodes a B(12)-independent methionine synthase, were isolated. Expression analysis confirmed that the B(12) riboswitch is a transcriptional regulator of metE in M. tuberculosis.Keywords
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