INDUCTION OF UNSCHEDULED DNA-SYNTHESIS IN PRIMARY CULTURE OF DOG, RAT, AND MOUSE UROTHELIAL CELLS BY ARYLAMINE AND NITROFURAN DERIVATIVES

  • 1 January 1982
    • journal article
    • research article
    • Vol. 42  (10) , 3974-3977
Abstract
The induction of unscheduled DNA synthesis in primary cultures of urothelial cells from rats, mice and dogs by nitrofurans and arylamines and their derivatives was examined. EM of the rat cell culture showed characteristics of epithelial cells. The cultured cells were exposed to the test chemicals in the serum-free medium containing 10 mM hydroxyurea and [3H]thymidine for 24 h, and unscheduled DNA synthesis was determined by autoradiography. N-Hydroxy-2-aminofluorene, N-hydroxy-2-acetylaminofluorene, N-hydroxy-4-aminobiphenyl and N-hydroxy-4-acetylaminobiphenyl were active in inducing unscheduled DNA synthesis in the urothelial cells of rats, mice and dogs. 2-Aminofluorene, N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide and 2-amino-4-(5-nitro-2-furyl)thiazole were active only in dog cells. 2-Acetylaminofluorene and 4-aminobiphenyl were not active. Urothelial cells apparently contain enzymes that can selectively activate bladder carcinogens and their metabolites.