High Speed HPLC Analysis of Polyamines in Plant Tissues
Open Access
- 1 February 1987
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 83 (2) , 232-234
- https://doi.org/10.1104/pp.83.2.232
Abstract
A high speed high performance liquid chromatography (HPLC) method for the quantification of putrescine, spermidine, and spermine in biological samples is described. The dansylation is followed by a sample cleanup. The isocratic HPLC-analysis with acetonitrile:H2O (72:28 volume/volume) on 10 centimeter long, 3 micrometer octadecyl silica columns (3 millimeter inner diameter) takes only 4.5 minutes. By our method about 100 analyses can be done in 1 day.Keywords
This publication has 3 references indexed in Scilit:
- POLYAMINESAnnual Review of Biochemistry, 1984
- Analysis of Polyamines in Higher Plants by High Performance Liquid ChromatographyPlant Physiology, 1982
- Determination of di-and polyamines by high-performance liquid chromatographic separation of their 5-dimethyl aminonaphthalene-1-sulfonyl derivativesJournal of Chromatography B: Biomedical Sciences and Applications, 1978