Determination of Ivermectin Residues in Meat and Liver by HPLC and Fluorometric Detection

Publisher Website
Google Scholar
Abstract
The method describes the analysis of ivermectin in meat and liver at a quantification limit of 5 μg/kg. This level is lower than the maximum residue limit (MRL) imposed for EEC countries. The absolute detection limit was 250 pg. The recoveries ranged from 70 % to 88 %. Ivermectin was extracted by a mixture of acetonitnie and water, purified on Bond Elut C18 columns and converted to a fluorescent derivative using trifluoroacetic anhydride and Nmethylimidazol. The analysis was performed on a liquid chromatograph fitted with a μ- Bondapak C18 column and ivermectin was detected by fluorescence spectroscopy. The method has already been used for routine analysis.

This publication has 10 references indexed in Scilit: