Liquid Chromatographic Retention Behavior and Separation of Chlorophenols on a -Cyclodextrin Bonded-Phase Column, Part I. Monoaromatic Chlorophenols: Retention Behavior

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Abstract
The liquid chromatographic retention behavior of nineteen monoaromatic chlorophenols on a β-cyclodextrin bondedphase column is investigated with respect to mobile phase composition, pH, temperature, and ionic strength. The mechanistic aspects of retention of these compounds on the β-cyclodextrin column are studied and compared to other reversed-phase columns. Most of the evidence suggests that the unique selectivity of this column is due to inclusion complex formation, which provides the physical basis for the resolution of positional isomers. Under certain chromatographic conditions, however, the more highly chlorinated congeners appear to be excluded from the cyclodextrin cavity; in such cases a normal-phase chromatographic mechanism is postulated, based on the interaction of the substrates with the secondary hydroxyls on the periphery of the cyclodextrin moieties.