Interactions between PII proteins and the nitrogenase regulatory enzymes DraT and DraG in Azospirillum brasilense
- 5 September 2006
- journal article
- Published by Wiley in FEBS Letters
- Vol. 580 (22) , 5232-5236
- https://doi.org/10.1016/j.febslet.2006.08.054
Abstract
In Azospirillum brasilense ADP‐ribosylation of dinitrogenase reductase (NifH) occurs in response to addition of ammonium to the extracellular medium and is mediated by dinitrogenase reductase ADP‐ribosyltransferase (DraT) and reversed by dinitrogenase reductase glycohydrolase (DraG). The PII proteins GlnB and GlnZ have been implicated in regulation of DraT and DraG by an as yet unknown mechanism. Using pull‐down experiments with His‐tagged versions of DraT and DraG we have now shown that DraT binds to GlnB, but only to the deuridylylated form, and that DraG binds to both the uridylylated and deuridylylated forms of GlnZ. The demonstration of these specific protein complexes, together with our recent report of the ability of deuridylylated GlnZ to be sequestered to the cell membrane by the ammonia channel protein AmtB, offers new insights into the control of NifH ADP‐ribosylation.Keywords
This publication has 25 references indexed in Scilit:
- Identification of Rhodospirillum rubrum GlnB Variants That Are Altered in Their Ability To Interact with Different Targets in Response to Nitrogen Status SignalsJournal of Bacteriology, 2006
- Effect of the over-expression of PII and PZ proteins on the nitrogenase activity of Azospirillum brasilenseFEMS Microbiology Letters, 2005
- ADP‐ribosylation of dinitrogenase reductase in Azospirillum brasilense is regulated by AmtB‐dependent membrane sequestration of DraGMolecular Microbiology, 2005
- Yeast Two-Hybrid Studies on Interaction of Proteins Involved in Regulation of Nitrogen Fixation in the Phototrophic BacteriumRhodobacter capsulatusJournal of Bacteriology, 2003
- AmtB Is Necessary for NH 4 + -Induced Nitrogenase Switch-Off and ADP-Ribosylation in Rhodobacter capsulatusJournal of Bacteriology, 2002
- Control of Nitrogenase Reactivation by the GlnZ Protein in Azospirillum brasilenseJournal of Bacteriology, 2001
- Effect of P II and Its Homolog GlnK on Reversible ADP-Ribosylation of Dinitrogenase Reductase by Heterologous Expression of the Rhodospirillum rubrum Dinitrogenase Reductase ADP-Ribosyl Transferase–Dinitrogenase Reductase-Activating Glycohydrolase Regulatory System in Klebsiella pneumoniaeJournal of Bacteriology, 2001
- Mutagenesis and Functional Characterization of the glnB , glnA , and nifA Genes from the Photosynthetic Bacterium Rhodospirillum rubrumJournal of Bacteriology, 2000
- The glnKamtB operonTrends in Genetics, 2000
- Regulation of nitrogen fixation in Azospirillum brasilenseFEMS Microbiology Letters, 1997