TECHNIQUE FOR MICROSCOPIC COUNT OF MICROORGANISMS DIRECTLY ON MEMBRANE FILTERS

Publisher Website
Google Scholar
Abstract
A technique is described for the quantitative detection of bacteria in liquid media. Bacterial suspensions are filtered through a membrane filter and consecutively stained for 2 to 5 minutes with a 110,000 dilution of the dye victoria pure blue directly on the filter. The filter is then dried for 10 minutes at 60 to 70[degree]C, and after the drying a drop of immersion oil is placed on the filter to make it transparent. Stained cells are counted under a conventional light microscope and for better differentiation an Eastman Kodak G-15 Wratten filter can be used with the light source. This method gave satisfactory results with Serratia marcescens and Bacillus globigii.