Single Multiplex Polymerase Chain Reaction To Detect Diverse Loci Associated with DiarrheagenicEscherichia coli
Open Access
- 1 January 2003
- journal article
- research article
- Published by Centers for Disease Control and Prevention (CDC) in Emerging Infectious Diseases
- Vol. 9 (1) , 127-131
- https://doi.org/10.3201/eid0901.010507
Abstract
We developed and tested a single multiplex polymerase chain reaction (PCR) that detects enterotoxigenic, enteropathogenic, enteroinvasive, and Shiga-toxin–producing Escherichia coli. This PCR is specific, sensitive, and rapid in detecting target isolates in stool and food. Because of its simplicity, economy, and efficiency, this protocol warrants further evaluation in large, prospective studies of polymicrobial substances.Keywords
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