Ribonuclease T1-Catalyzed Synthesis of Triribonucleoside Diphosphates Having Guanosine Residue at the 5'-End

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Abstract
The reactivity of several ribonucleosides with guanosine-2', 3' cyclic phosphate under catalysis by ribonuclease T1 [EC 2. 7. 7. 26] was estimated and it was found that the reactivity decreased depending on the nucleoside used in the order of cytidine>uridine>inosine>adenosine. In the similar reaction between guanosine-2', 3' cyclic phosphate and several diribonucleoside monophosphates, the largest reaction rate was observed when the diribonucleoside monophosphates containing cytidine at their 5'-end were used. The nature of nucleoside residue at 3'-end of the dinucleoside monophosphate also influenced on the reaction rate. Thus, cytidylyl-(3', 5')-cytidine was most reactive, while cytidylyl-(3', 5')-guanosine reacted very slowly. Using this reaction, twelve triribonucleoside diphosphates, which have a guanylyl residue at their 5'-end were prepared and the products were characterized and were pure enough to use as coding triplets.