DEMONSTRATION OF CIRCULATING 1,24,25-TRIHYDROXYVITAMIN D3IN MAN BY RADIOIMMUNOASSAY
- 1 April 1982
- journal article
- research article
- Published by Wiley in Clinical Endocrinology
- Vol. 16 (4) , 337-343
- https://doi.org/10.1111/j.1365-2265.1982.tb00725.x
Abstract
1,24,25-trihydroxyvitamin D3 has been detected in human serum using a sensitive radioimmunoassay. Tritiated 1,24,25-trihydroxyvitamin D3 was synthesized biologically and used as tracer to monitor the recovery of endogenous metabolite during isolation from serum. Circulating 1,24,25(OH)3D3 in normal subjects ranged from 9·3 to 18·5 pmol/l but was not detectable (> 2·3 pmol/l) in serum from nephrectomized subjects. The trihydroxy metabolite was elevated in three out of four vitamin D deficient subjects who were being treated with vitamin D3.This publication has 15 references indexed in Scilit:
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