The preparation of protoplasts fromAspergillus fumigatusmycelium

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Abstract
Protoplasts have been prepared from the mycelium of Aspergillus fumigatus, using a lytic enzyme mixture from Trichoderma harzianum. A variety of experimental conditions were investigated in order to achieve optimal conditions for protoplast production. Electron micrographs showed the preparations to be free of cell-wall. Material obtained by this procedure can be used as a source of cytoplasmic antigens for further analysis. Aspergillus fumigatus Protoplasten wurden durch Behandlung der Mycelien mit einer enzymatischen Mischung von Trichoderma harzianum erhalten. Elektronenmikroskopisch erwiesen sich die Protoplasten als frei von Zellwandmaterial. Die cytoplasmatischen Antigene der Protoplasten werden zur weiteren Forschung verwendet.