Involvement of an Inducible Cytochrome P-450 in Precocene II Oxidation by Streptomyces Griseus
- 1 January 1989
- journal article
- research article
- Published by Taylor & Francis in Biocatalysis
- Vol. 2 (2) , 151-160
- https://doi.org/10.3109/10242428909003656
Abstract
Streptomyces griseus oxidizes the insecticide precocene II to its cis- and trans-dihydrodiols and 3-chromenol after growth on an enriched medium containing soybean flour. Oxidation of precocene II is dependent on the level of cytochrome P-450 in this organism. Extracts of cells grown on media lacking soybean flour were devoid of cytochrome P-450 and could not oxidize precocene II. In an in vitro reconstituted system containing NADPH, spinach ferredoxin reductase, spinach ferredoxin and ammonium sulfate fractions enriched in cytochrome P-450, precocene II was oxidized to its dihydrodiols. An aerial mycelium-negative variant of S. griseus (AMY mutant), that was unable to elicit cytochrome P-450 when grown on soybean flour-enriched medium, failed to oxidize precocene II.Keywords
This publication has 10 references indexed in Scilit:
- Hepatotoxicity of precocene I in ratsBiochemical Pharmacology, 1987
- Induction of cytochrome P-450 in Streptomycesgriseus by soybean flourBiochemical and Biophysical Research Communications, 1986
- Comparative studies on pharmacokinetics and metabolism of the anti‐juvenile hormone precocene IIArchives of Insect Biochemistry and Physiology, 1985
- Resolution and assignement of absolute configuration to the (+)- and (−)- and 3,4-diol metabolites of the anti-juvenile hormone precocene ITetrahedron Letters, 1982
- Hepatotoxicity of the anti-juvenile hormone precocene II and the generation of dihydrodiol metabolitesChemico-Biological Interactions, 1981
- Precocene II metabolism: Comparative in vivo studies among several species of insects, and structure elucidation of two major metabolitesPesticide Biochemistry and Physiology, 1980
- Radiosynthesis and metabolism of the insect antijuvenile hormone, precocene IIJournal of Agricultural and Food Chemistry, 1977
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Microbial transformations of antitumor compounds. 1. Conversion of acronycine to 9-hydroxyacronycine by Cunninghamella echinulataJournal of Medicinal Chemistry, 1974