Temperature Programming in Open Tubular Liquid Chromatography
- 1 July 1996
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography & Related Technologies
- Vol. 19 (13) , 2089-2099
- https://doi.org/10.1080/10826079608017143
Abstract
We have examined the separation of test mixtures by linear and step temperature gradients in open tubular capillary liquid chromatography, in reverse and normal-phase modes. Separations using temperature gradients with the upper temperature limit of 433 K were compared to isothermal runs in terms of chromatographic efficiency, analysis time and selectivity. When a temperature gradient (363 K to 433 K in 12 min) was utilized in reverse phase separation of chlorobenzenes, a 40% reduction in analysis time, compared to an isothermal separation at 363 K, was accomplished.Keywords
This publication has 26 references indexed in Scilit:
- Highly efficient separations on 5 μm internal diameter open tubular capillaries coated with thick polyacrylate stationary phasesChromatographia, 1995
- Performance of an ethoxyethylacrylate stationary phase for open-tubular liquid chromatographyJournal of Chromatography A, 1995
- Preparation of small diameter thick film capillary columns for microcolumn separationsJournal of Microcolumn Separations, 1994
- Study of polyorganosiloxanes (native and solvent swollen) for the preparation of narrow (5–15 μm I.D.) and long (1–6 m) open tubular columns in reversed-phase liquid chromatographyJournal of Chromatography A, 1993
- Improved ultraviolet detection in high-temperature open-tubular liquid chromatographyJournal of Chromatography A, 1993
- Reversed- and normal-phase separations by high-temperature open-tubular column liquid chromatographyJournal of Chromatography A, 1992
- High-temperature open-tubular capillary column liquid chromatographyJournal of Chromatography A, 1992
- Rapid analysis of proteins and peptides by reversed-phase chromatographyJournal of Chromatography A, 1987
- Microcapillary liquid chromatography in open tubular columns with diameters of 10–50 μm : Potential application to chemical ionization mass spectrometric detectionJournal of Chromatography A, 1981
- Studies of open-tubular micro-capillary liquid chromatography: II. Chemically bonded octadecylsilane stationary phaseJournal of Chromatography A, 1978