Subcellular Distribution of Aspartate Transaminase, Alanine Amino Transferase, Glutamate Dehydrogenases, and Lactate Dehydrogenase in Tetrahymena*
- 1 May 1972
- journal article
- research article
- Published by Wiley in The Journal of Protozoology
- Vol. 19 (2) , 375-378
- https://doi.org/10.1111/j.1550-7408.1972.tb03481.x
Abstract
SYNOPSIS. Mitochondria and peroxisomes were isolated from homogenates of Tetrahymena pyriformis by sedimentation through a sucrose gradient. Succinate dehydrogenase was used as a mitochondrial marker; catalase and isocitrate lyase were used to mark the peroxisomal fraction. Lactate dehydrogenase, glutamate dehydrogenase, and alanine aminotransferase were found only in the mitochondrial fraction. Aspartate transaminase was found in both mitochondrial and peroxisomal fractions.Keywords
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