Cell differentiation in Dictyostelium under submerged conditions.

Abstract
Previously, it has not been possible to obtain spore and stalk cell differentiation of the cellular slime molds in submerged cultures. It is shown that cells, when placed in roller tubes under an atmosphere of O2, will form clumps and differentiate in 48-72 h into mature spores and stalk cells. Although differentiation occurs without the normal morphogenetic movements, there is the appearance of an anteroposterior polarity of the cells in the clump. In addition to O2 several other factors were examined that affect differentiation.