A method for continuous preparative electrophoresis using a supporting medium
- 1 February 1981
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 2 (1) , 39-45
- https://doi.org/10.1002/elps.1150020106
Abstract
A method for continuous preparative electrophoresis is presented. It is based on the principle that a supporting medium, e.g. a polyacrylamide gel, is moved continuously relative to an electrical field. At one fixed point, the sample is applied to the gel. The charged particles migrate with different velocities in the supporting medium. Simultaneously, they are dragged along with the supporting medium. This procedure leads to a two‐dimensional separation of charged particles, which is necessary for a continuous operation of an electrophoresis apparatus. Three different designs of apparatuses, using this method, are presented in this paper. The operation of one construction was tested by separating three protein mixtures.Keywords
This publication has 14 references indexed in Scilit:
- Studies on preparative electrophoresis apparatus: Some eminent advantages of the elution chamber sandwiched between gel column halvesAnalytical Biochemistry, 1979
- Free-Flow Electrophoresis I. Theoretical and Experimental Investigations of the Influence of Mechanical and Electrokinetic Variables on the Efficiency of the MethodHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1975
- Continuous preparative gel electrophoresisAnalytical Biochemistry, 1973
- A simplified and efficient system for separating proteins by preparative polyacrylamide electrophoresisAnalytical Biochemistry, 1971
- Apparatus for large-scale preparative polyacrylamide gel electrophoresisAnalytical Biochemistry, 1969
- An apparatus for preparative polyacrylamide electrophoresis: The isolation of a ribonuclease inhibitorAnalytical Biochemistry, 1969
- Simplified technique for preparative disc electrophoresisAnalytical Biochemistry, 1967
- Separation and Recovery of Proteins by Elution-ConvectionSeparation Science, 1966
- Eine Neuentwicklung der trägerfreien kontinuierlichen Elektrophorese. Zur Trennung hochmolekularer und grobdisperser TeilchenHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1964
- Preparative methods for disk electrophoresis with special reference to the isolation of pituitary hormonesAnalytical Biochemistry, 1963