Adsorption and expression of penicillin G acylase immobilized onto methacrylate polymers generated with varying pore generating solvent volume
- 1 September 1991
- journal article
- Published by Springer Nature in Applied Biochemistry and Biotechnology
- Vol. 30 (3) , 297-302
- https://doi.org/10.1007/bf02922033
Abstract
Adsorption and expression of penicillin G acylase was studied on macroporous methacrylate polymer beads of differing pore volume, generated with kerosene. The absorption and expression of the penicillin G acylase was dependent on pore volume. Maximum expression of 57% of adsorbed enzyme was obtained on beads synthesized with 40 mL of kerosene, indicating minimum pore-diffusion limitations.Keywords
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