Peptide display on filamentous phage capsids An new powerful tool to study protein—ligand interaction
- 27 July 1992
- journal article
- review article
- Published by Wiley in FEBS Letters
- Vol. 307 (1) , 66-70
- https://doi.org/10.1016/0014-5793(92)80903-t
Abstract
Peptides can be displayed on the surface of filamentous bacteriophages by fusion to phage coat proteins. It was recently shown that vast (108) collections of phages, each exposing a variant of the original peptide, can be constructed and utilized as a general source of peptide ligands. By panning these libraries on a target molecule linked to a solid support it is possible to select, out of the hundreds of millions of clones, those few phages that display a peptide that binds the target molecule. Searching these libraries is a powerful tool to be applied in many areas of fundamental and applied biology.Keywords
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