EFFECT OF LONG-TERM ADMINISTRATION OF 4-(DIMETHYLAMINO)AZOBENZENE ON LIVER-CELL LINE DERIVED FROM RATS FED THIS CHEMICAL

  • 1 January 1976
    • journal article
    • research article
    • Vol. 67  (6) , 879-883
Abstract
Liver tissue cells derived from rats fed 4-(dimethylamino)azobenzene (DAB) were maintained in vitro for several months. These cells showed higher malignancy after treatment with 4.8 or 19.2 .mu.g/ml of DAB. S.c. back transplantation of DAB-treated cells showed an earlier appearance of palpable tumors and a higher rate of take than nontreated control cells. The tumor size at 60 days after back transplantation was also significantly larger in DAB-treated cells. Long-term treatment with DAB produced few morphological changes of cells in vitro, but the plating efficiency and resistance to toxic effects of DAB were significantly higher in treated cells than control cells. No definite differences in chromosome number were found between treated and control cells. Cell malignancy could be promoted by long-term treatment with DAB in vitro.