Control of Gaseous Environment of Organisms using a Diffusion Column Technique

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Abstract
A technique based on the theory of steady-state diffusion of gases in a long column provides a simple means of controlling the gas concentration in biological experiments. The diffusion column consists of a sand-filled tube with side-arms along its length. Selected gas concentrations are applied at both ends of the column and maintain a predictable concentration gradient along the column. The gas composition at each side-arm is thus known and can be adjusted by alteration of the controlling concentrations at the ends of the column. The response to gas concentration of small bodies such as seeds and micro-organisms can be tested by placing them in small cuvettes attached to the side-arms. The technique is well suited for experiments needing a closely graded series of gaseous concentrations. The design and use of an electrode to monitor oxygen concentration is also described.

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