Analysis of a Growth-Promoting Factor forBabesia caballiCultivation
- 1 December 2001
- journal article
- Published by American Society of Parasitologists in Journal of Parasitology
- Vol. 87 (6) , 1484-1486
- https://doi.org/10.1645/0022-3395(2001)087[1484:aoagpf]2.0.co;2
Abstract
Serum-free media were examined to culture Babesia caballi. Daigo's T (DT) basal medium supplemented with Daigo's GF21 (GF21) or GIT medium, which already contains GF21, supported the parasite propagation at 37 C in a humidified atmosphere under 5% CO2 in air. Growth of B. caballi was dependent of the suitable concentration (10-20%) of GF21. Therefore, GF21 was suggested as the growth-promoting factor for B. caballi. However, GIT medium did not support the growth of parasites from cryopreserved stabilates, and serum supplementation was essential for the retrieval of parasites.Keywords
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