Expresion of therpsOandpnpgenes: structural analysis of a DNA fragment carrying their control regions
Open Access
- 1 January 1984
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (15) , 6091-6099
- https://doi.org/10.1093/nar/12.15.6091
Abstract
Precise physical mapping of the genes rpsO and pnq coding respectively for ribosomal protein S15 and polynucleotide phosphorylase together with regions involved in the regulation of their expression has been obtained by the analysls of in vitro deletion mutants. The results suggest that each gene has its own promotor, but that there is coexpression of rpsO and pnp. The nucleotide sequence of rpsO and of the beginning of pnp is presented and includes the presumed regulatory regions of these genes. Several features of the sequence support the mapping experiments and are discussed in relation to the expression of the ribosomal and pnp genes.Keywords
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