A catalytic role for threonine‐12 of E. coli asparaginase II as established by site‐directed mutagenesis
- 8 July 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 285 (1) , 55-58
- https://doi.org/10.1016/0014-5793(91)80723-g
Abstract
A threonine‐12 to alanine mutant of E. coli asparaginase II (EC 3.5.1.1) has less than 0.01% of the activity of wild‐type enzyme. Both tertiary and quaternary structure of the enzyme are essentially unaffected by the mutation; thus the activity loss seems to be the result of a direct impairment of catalytic function. As aspartate is still bound by the mutant enzyme, Thr‐12 appears not be involved in substrate binding.Keywords
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