Visualization of Polymercurimethane-Labeled fd Bacteriophage in the Scanning Transmission Electron Microscope
- 21 December 1979
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 206 (4425) , 1419-1421
- https://doi.org/10.1126/science.92053
Abstract
Each of the 2700 coat proteins of fd bacteriophage was labeled with tetrakis(acetoxymercuri)methane (TAMM) or aquoglycylmethionineplatinum(II). The TAMM-labeled specimens reveal striking bright spots in the scanning transmission electron microscope which arise from clustering. Measurements of mass show increases consistent with the addition of four mercury atoms or one platinum atom, respectively, to each coat protein.Keywords
This publication has 6 references indexed in Scilit:
- Tetrakis(acetoxymercuri)methane: a polymetallic reagent for labeling sulfur in nucleic acids.Proceedings of the National Academy of Sciences, 1978
- Hydrodynamic properties and structure of fd virusJournal of Molecular Biology, 1977
- [36] Reactions with N-ethylmaleimide and p-mercuribenzoatePublished by Elsevier ,1972
- The Chemical Modification of Biopolymers. III. The Introduction of SH Groups into Taka-amylase ABulletin of the Chemical Society of Japan, 1970
- Filamentous bacterial virusesMicrobiology and Molecular Biology Reviews, 1969
- Introduction of sulfhydryl groups into proteins using acetylmercaptosuccinic anhydrideArchives of Biochemistry and Biophysics, 1962