Rapid synthesis of oligodeoxyribonucleotides on a grafted polymer support
Open Access
- 1 January 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 6 (6) , 2041-2056
- https://doi.org/10.1093/nar/6.6.2041
Abstract
Rapid synthesis of oligodeoxyribonucleotides is described by a solid phase method. Polyaterene grafted on the surface of polytetrafluoroethylene has been found to be an adequate support. The properties of the support were studied in the synthesis of a number of oligonucleotides 6 t o 15 nucleotides long. A flow-type semi-automatic synthesizer has been used. Each nucleotide addition involves seven steps and takes 8 hours. Oligonucleotide isolated and purified by ion-exchange chromatography amounts to 1–3 μmol per gram of support.Keywords
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